Detection of viable Salmonella Typhi by reverse transcription-multiplex polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses
Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...
متن کاملSpecific detection of Salmonella spp. by multiplex polymerase chain reaction.
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria. In addition to the phoP primers, the Hin and the H-1i primers, which tar...
متن کاملDetection of three avian respiratory viruses by single-tube multiplex reverse transcription-polymerase chain reaction assay.
Acute respiratory tract infections are leading causes of morbidity in poultry farms throughout the world. Avian pneumovirus (APV), avian influenza virus (AIV), and Newcastle disease virus (NDV) have been recognized as the most important pathogens of both chicken and turkeys. Single-virus reverse transcription-polymerase chain reaction (sRT-PCR) assays are used extensively to detect these viruse...
متن کاملsimultaneous and rapid detection of salmonella typhi, bacillus anthracis, and yersinia pestis by using multiplex polymerase chain reaction (pcr)
conclusion the designed methods are accurate, rapid, and inexpensive to find and differentiate these bacteria from similar bacteria. they can be applied for rapid diagnosis of these agents in different specimens, and bioterrorism cases. background salmonella typhi, bacillus anthracis, and yersinia pestis are some serious human pathogens, which their early diagnosis is of great importance. salmo...
متن کاملSimultaneous and Rapid Detection of Salmonella typhi, Bacillus anthracis, and Yersinia pestis by Using Multiplex Polymerase Chain Reaction (PCR)
BACKGROUND Salmonella typhi, Bacillus anthracis, and Yersinia pestis are some serious human pathogens, which their early diagnosis is of great importance. Salmonella typhi, Bacillus anthracis, and Yersinia pestis cause typhoid fever, anthrax, and plague respectively. These bacteria can be used to make biologic weapons. OBJECTIVES In this study, we designed a new and rapid diagnostic method ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Emirates Journal of Food and Agriculture
سال: 2017
ISSN: 2079-052X
DOI: 10.9755/ejfa.2016-12-1867