Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR
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چکیده
منابع مشابه
Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays.
A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...
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متن کاملStrawberry Necrotic Shock Virus: A New Virus Previously Thought to Be Tobacco Streak Virus
Tobacco streak virus (TSV) has a wide host range that exceeds 80 species (Fulton, 1948). Most of the efforts carried out previously comparing TSV isolates was based on immunological relations between them. The isolates of the virus from Fragaria and Rubus have been considered to be very closely related if not identical while they are distinct from isolates of other hosts. While trying to charac...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2016
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2016.06.005