Detection of Serum Antibody to Infectious Bovine Rhinotracheitis Virus by Enzyme Linked Immunosorbent Assay

Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus

The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...

Enzyme-linked immunosorbent assay for serology of infectious bovine rhinotracheitis virus infections.

An enzyme-linked immunosorbent assay was developed to detect antibodies to infectious bovine rhinotracheitis virus in bovine sera. The test used viral antigen purified in a sucrose density gradient, and the antibody titers detected were up to 100 times higher than those seen in the serum neutralization test. When 250 sera were tested by those two tests, 146 were positive and 49 were negative in...

Antibody assay for Japanese encephalitis virus in bovine serum by enzyme-linked immunosorbent assay (ELISA).

standardization of an enzyme-linked immunosorbent assay for detection of infectious bronchitis virus antibody.

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...