Detection of respiratory bacterial pathogens causing atypical pneumonia by multiplex Lightmix ® RT-PCR
نویسندگان
چکیده
منابع مشابه
Identification of Pathogens in Nasopharyngeal Secretions of Patients with and without Pneumonia by Multiplex RT-PCR Method
Background and purpose: Pneumonia is the most common infectious cause of death. This study aimed at investigating the causative agent of pneumonia in nasopharyngeal secretions by PCR method. Materials and methods: This case-control study was carried out in patients (older than 19 years of age) suspected of pneumonia admitted to Qaemshahr Razi Hospital and Sari Imam Khomeini Hospital...
متن کاملDetection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
OBJECTIVE The aim of this study was to determine the etiology and prevalence of pediatric CAP in Beijing using a real-time polymerase chain reaction (PCR) technique. METHODS Between February 15, 2011 and January 18, 2012, 371 pediatric patients with CAP were enrolled at Beijing Children's Hospital. Sixteen respiratory viruses and two bacteria were detected from tracheal aspirate specimens usi...
متن کاملAtypical pathogens causing community-acquired pneumonia in adults.
OBJECTIVE To determine the frequency of community-acquired respiratory pathogens with special focus on atypical organisms in patients presenting to a tertiary care facility with community-acquired pneumonia (CAP). METHODS The descriptive study on adult patients was conducted from February 2007 to March 2008 at the Aga Khan University Hospital, Karachi. It comprised 124 consenting patients of ...
متن کاملSimultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry
Citation: Zhang C, Xiu L, Xiao Y, Xie Z, Ren L and Peng J (2018) Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry. Front. Cell. Infect. Microbiol. 8:107. doi: 10.3389/fcimb.2018.00107 Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass S...
متن کاملThe role of multiplex PCR test in identification of bacterial pathogens in lower respiratory tract infections
OBJECTIVES Lower respiratory tract infection is one of the most important causes of morbidity and mortality. However establishing a microbial diagnosis for patients with lower respiratory tract infection is still challenging and is often achieved in only half of cases by conventional methods. This study was designed to compare the fast responsive PCR method with the culture method in lower resp...
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ژورنال
عنوان ژورنال: International Journal of Medical Microbiology
سال: 2018
ISSN: 1438-4221
DOI: 10.1016/j.ijmm.2018.01.010