Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor

The acquisition of new mobile genetic elements contributes to the diversity Vibrio cholerae strains. An important role in this process belongs material obtained from phages. aim work was identify phage-induced PLE islands strains V. O1 serogroup and determine resistance isolates with without those lytic activity diagnostic cholera El Tor bacteriophage. Materials methods . Whole genomes nucleotide sequences toxigenic non-toxigenic presented NCBI GenBank were used for work. Bioinformatic analysis performed using BLAST algorithm, MEGA X (or BioEdit v. 7.0.9.0). test phages carried out according A. Gratia technique. Results discussion 39 imported territory Russian Federation neighboring countries has revealed one strain classical biovar containing PLE5 island, 13 PLE4 island. have not been found It is shown that belong genovariants ctxB1 gene allele. Isolates element caused sporadic cases disease 1994–1999, as well outbreaks (in 1993–1994, 1998 – Dagestan, 1993 Tatarstan) Ukraine (1994–1995). suggested that, perhaps, presence island makes a certain contribution phage (55.6 % phage-resistant detected), but there are other mechanisms yet identified. Thus, data on genome earlier O1, obtained, which expands information about their organization.