Detection of Goss’s Wilt Pathogen Clavibacter michiganensis subsp. nebraskensis in Maize by Loop-Mediated Amplification
نویسندگان
چکیده
منابع مشابه
Goss's Bacterial Wilt Development and Clavibacter michiganensis subsp. nebraskensis Interactions with Spray Adjuvants
..............................................................................................53 Introduction..........................................................................................54 Methods and Materials..............................................................................57 Results.........................................................................................
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Clavibacter michiganensis subsp. nebraskensis (Cmn), the causal organism of Goss's wilt and leaf blight of maize, can be detected in the phyllosphere of its host prior to disease development. We compared the morphology and pathogenicity of 37 putative isolates of Cmn recovered from asymptomatic and symptomatic maize leaves. Thirty-three of the isolates produced mucoid orange colonies, irrespect...
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Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here...
متن کاملLinear plasmid in the genome of Clavibacter michiganensis subsp. sepedonicus.
Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.
متن کاملSpecific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains
Accurate and rapid detection of bacterial plant pathogen is the first step toward disease management and prevention of pathogen spread. Bacterial plant pathogens Clavibacter michiganensis subsp. nebraskensis (Cmn), Pantoea stewartii subsp. stewartii (Pss), and Rathayibacter tritici (Rt) cause Goss's bacterial wilt and blight of maize, Stewart's wilt of maize and spike blight of wheat and barley...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2016
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-10-15-0249-r