Detection of Gaeumannomyces graminis Varieties Using Polymerase Chain Reaction with Variety-Specific Primers

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Detection of Babesia ovis Using Polymerase Chain Reaction.

Detection of Erysiphe necator in Air Samples Using the Polymerase Chain Reaction and Species-Specific Primers.

ABSTRACT A polymerase chain reaction (PCR) assay employing species-specific primers was developed to differentiate Erysiphe necator from other powdery mildews common in the northwest United States. DNA was extracted from mycelia, conidia, and/or chasmothecia that were collected from grape leaves with a Burkard cyclonic surface sampler. To differentiate E. necator from other erysiphaeceous fungi...

Detection and Differentiation of Phaeoisariopsis griseola Isolates with the Polymerase Chain Reaction and Group-Specific Primers

Angular leaf spot (ALS) disease of common bean (Phaseolus vulgaris L.) is caused by the imperfect fungus Phaeoisariopsis griseola (Sacc.) Ferraris. ALS is an important disease in tropical and subtropical countries and can cause severe bean yield losses (approximately 40 to 80%) under favorable environmental conditions (5,7,19,20). Breeding for disease resistance is the most practical approach f...

NEW PRIMERS FOR DETECTION OF Leishmania infantumUSING POLYMERASE CHAIN REACTION

Leishmania infantum causes visceral leishmaniasis (VL) in the New World. The diagnosis of VL is confirmed by parasitological and serological tests, which are not always sensitive or specific. Our aim was to design new primers to perform a Polymerase Chain Reaction (PCR) for detecting L. infantum. Sequences of the minicircle kinetoplast DNA (kDNA) were obtained from GenBank, and the FLC2/RLC2 pr...