Detection of Erysiphe necator in Air Samples Using the Polymerase Chain Reaction and Species-Specific Primers

Detection of Erysiphe necator in Air Samples Using the Polymerase Chain Reaction and Species-Specific Primers.

ABSTRACT A polymerase chain reaction (PCR) assay employing species-specific primers was developed to differentiate Erysiphe necator from other powdery mildews common in the northwest United States. DNA was extracted from mycelia, conidia, and/or chasmothecia that were collected from grape leaves with a Burkard cyclonic surface sampler. To differentiate E. necator from other erysiphaeceous fungi...

Species-specific Polymerase Chain Reaction Primers for Detecting Prevotella nigrescens

A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop Prevotella nigrescens-specific polymerase chain reaction (PCR) primers. The specificity was tested against 10...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Detection of Babesia ovis Using Polymerase Chain Reaction.