Detection of Equine Rotavirus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
نویسندگان
چکیده
منابع مشابه
Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Zika virus (ZIKV) has emerged as a major global public health concern in the last two years due to its link as a causative agent of human birth defects. Its rapid expansion into the Western Hemisphere as well as the ability to be transmitted from mother to fetus, through sexual transmission and possibly through blood transfusions has increased the need for a rapid and expansive public health re...
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The aim of this study was to produce a sensitive, simple and rapid diagnostic method for the detection of foot-and-mouth disease virus (FMDV) in suspected cases of FMD, a one-step single-tube method of reverse transcription loop-mediated isothermal polymerase amplification (RT-LAMP). A set of six common primers was designed to target the highly conserved region of the RNA polymerase 3Dpol gene ...
متن کاملReverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus
BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...
متن کاملA reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملOne-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV
Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2010
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.09-0446