Detection of Entamoeba histolytica \E.dipar and Ascaris lumbricoides in fresh vegetables consumed collected randomly farms from Brack Al-shati, Libya

application of multiplex pcr for detection and differentiation of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii.

background: entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica . multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. methods: for detection and differentiation of the three-microscopy indistinguishable entamoeba...

PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.

This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is t...

Ascaris Lumbricoides

PRELIMINARY COMPARATIVE STUDY OF ENTAMOEBA HISTOLYTICA AND ENTAMOEBA DISPAR BY PCR TECHNIQUE IN IRAN

It has been suggested that two distinct species exist within what was originally known as Entamoeba histolytica and E. dispar. These are Entamoeba dispar for the nonpathogenic and E. histolytica for the pathogenic fonn. Differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both fonns can infect the human intestinal cavity...

The cultivation of entamoeba histolytica in HSr+ s medium

  In 1926 for the first time Dobell and laid low synthesized and used Hsre+ s ( horse serum, ringer, egg+ starch rice) as a culture medium for growth of E.histolytica. since then this medium along with tens of other media have been used for growth of E.histolytica and other intestinal microorganisms. This medium has been used worldwide in many diagnostic and research laboratories. In Iran, in ...