Detection of Cryptococcus neoformans Gene Using PCR.
نویسندگان
چکیده
منابع مشابه
DETECTION OF CRYPTOCOCCUS NEOFORMANS BY SEMINESTED PCR IN CEREBROSPINAL FLUID
ABSTRACT Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years. Cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (CNS) in immunocompetent as well as immunocompromised patients. We report the development of a semi-nested- PCR-based assay for the ...
متن کاملdetection of cryptococcus neoformans by seminested pcr in cerebrospinal fluid
abstract life-threatening infections caused by the encapsulated fungal pathogen cryptococcus neoformans have been increasing steadily over the past 10 years. cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (cns) in immunocompetent as well as immunocompromised patients. we report the development of a semi-nested- pcr-based assay for the d...
متن کاملDevelopment of a nested PCR for detection of Cryptococcus neoformans in cerebrospinal fluid.
We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conve...
متن کاملCryptococcus neoformans.
Seven nonencapsulated mutants of Cryptococcus neoformans were isolated from an encapsulated strain of human origin. Initially, the mutants were avirulent for mice. After several months of subculturing, six of the seven isolates reverted LO the encapsulated state and possessed varying degrees of virulence. The results of these experiments suggest that a strong correlation exists between the pres...
متن کاملEcology of Cryptococcus neoformans.
Cryptococcus neoformans is a basidiomycetous, yeast-like fungus which, following inhalation from an environmental source, causes respiratory and neurological disease in humans and animals. Though the ecology of C. neoformans is poorly understood, recent observations provide clues about its life-cycle in nature. Two biotypes of C. neoformans are recognised, C. neoformans var. neoformans (serotyp...
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ژورنال
عنوان ژورنال: Nippon Ishinkin Gakkai Zasshi
سال: 1994
ISSN: 0916-4804,1882-0476
DOI: 10.3314/jjmm.35.141