Detection of Brachyspira hyodysenteriae, Lawsonia intracellularis and Brachyspira pilosicoli in feral pigs
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منابع مشابه
Detection of Brachyspira hyodysenteriae, Lawsonia intracellularis and Brachyspira pilosicoli in feral pigs.
Feral pigs are recognized as being a potential reservoir of pathogenic microorganisms that can infect domestic pigs and other species. The aim of this study was to investigate whether feral pigs in Western Australia were colonized by the pathogenic enteric bacteria Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli. A total of 222 feral pigs from three study-popu...
متن کاملApplication of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs.
The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially av...
متن کاملImmunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces.
Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions. The purpose of the present study was to compare the relative sensitivi...
متن کاملAntimicrobial susceptibility of porcine Brachyspira hyodysenteriae and Brachyspira pilosicoli isolated in Sweden between 1990 and 2010
BACKGROUND The anaerobic spirochetes Brachyspira hyodysenteriae and Brachyspira pilosicoli cause diarrheal diseases in pigs. Their fastidious nature has hampered standardization of methods for antimicrobial susceptibility testing. For monitoring of antimicrobial susceptibility wild type cutoff values are needed to define where the wild type distribution of MICs ends and no approved cutoffs are ...
متن کاملRapid Detection of Brachyspira Hyodysenteriae and Lawsonia Intracellularis in Swine Faecal and Mucosal Specimens by Multiplex Pcr
Multiplex polymerase chain reaction (M-PCR) was developed to determine its usefulness as a rapid and specific method for routine diagnosis of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in pigs. The assay was based on simultaneous amplification of the tlyA-encoded haemolysin gene of B. hyodysenteriae and 16S rRNA sequences of L. intracellularis. The results of this study ...
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ژورنال
عنوان ژورنال: Veterinary Microbiology
سال: 2009
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2008.08.006