Detection of Antibody to Japanese Encephalitis Virus (JEV) by Enzyme-Linked Immunosorbent Assay (ELISA).

Antibody assay for Japanese encephalitis virus in bovine serum by enzyme-linked immunosorbent assay (ELISA).

Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

detection of human bloodstains by enzyme-linked immunosorbent assay (elisa)

the microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (elisa) , usi ng alkaline phosphatase anti-human i gg conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed fr om human and laborat or y anima l s i n t he form of small dr ied b loodst a i ns on f ilt e r paper . the modi f i e d elisa t e chnique w...

Labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detection of Japanese encephalitis antibody in swine sera.

Labeled avidin-biotin (LAB) enzyme-linked immunosorbent assay (ELISA) was applied to detect Japanese encephalitis OE) antibody in swine sera. Purified laGAr-01 vaccine of lE, biotin labeled anti-swine Ig or IgM rabbit IgG conjugate and horseradish peroxidase (HRP) were employed in the assay. The f1 chain specific anti-IgM conjugate could detect only IgM antibody, but the anti-Ig conjugate could...

Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus

The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...