Detection of a phosphorylated glycine-serine linker in an IgG-based fusion protein
نویسندگان
چکیده
منابع مشابه
Detection of a phosphorylated glycine-serine linker in an IgG-based fusion protein
Molecular mass determination by electrospray ionization mass spectrometry of a recombinant IgG-based fusion protein (mAb1-F) produced in human embryonic kidney (HEK) cells demonstrated the presence of a dominant +79 Da product variant. Using LC-MS tryptic peptide mapping analysis and collision-induced dissociation (CID) and electron-transfer/higher-energy collision dissociation fragmentations, ...
متن کاملDetermining protein-protein interactions by oxidative cross-linking of a glycine-glycine-histidine fusion protein.
The Ni(II) complex of the tripeptide NH2-glycine-glycine-histidine-COOH (GGH) mediates efficient protein-protein cross-linking in the presence of oxidants such as oxone and monoperoxyphthalic acid (MMPP). Here we demonstrate that GGH fused to the amino terminus of a protein can still support cross-linking. The tripeptide was expressed at the amino terminus of ecotin, a dimeric macromolecular se...
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چکیده ندارد.
15 صفحه اولTwo Dimensional Structural Analysis and Expression of a New Staphylococcus aureus Adhesin Based Fusion Protein
Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA)...
متن کاملLINKER: a program to generate linker sequences for fusion proteins.
The construction of functional fusion proteins often requires a linker sequence that adopts an extended conformation to allow for maximal flexibility. Linker sequences are generally selected based on intuition. Without a reliable selection criterion, the design of such linkers is often difficult, particularly in situations where longer linker sequences are required. Here we describe a program c...
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ژورنال
عنوان ژورنال: mAbs
سال: 2016
ISSN: 1942-0862,1942-0870
DOI: 10.1080/19420862.2016.1236165