Detection and Identification of Infectious Hematopoietic Necrosis Virus (IHNV) by Reverse Transcription (RT)-polymerase Chain Reaction (PCR)
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چکیده
منابع مشابه
Simultaneous Detection of Infectious Hematopoietic Necrosis Virus (IHNV) and Infectious Pancreatic Necrosis Virus (IPNV) by Reverse Transcription (RT)-Polymerase Chain Reaction (PCR)
Multiple virus infections have recently been observed during routine examination of salmonid fish.1) Infectious hematopoietic necrosis virus (IHNV) and infectious pan creatic necrosis virus (IPNV) are often isolated together or with other viruses, such as a herpes virus or a retro-like virus.1,2) In case of multiple virus infections, only the virus which replicates most efficiently will be dete...
متن کاملReverse transcriptase-polymerase chain reaction (rt-PCR).
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
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Infectious bronchitis (IB) is a highly contagious disease of the respiratory and urogenital tract of chickens, caused by infectious bronchitis virus (IBV), a member of the family Coronaviridae. The disease is common throughout the world where chickens are produced commercially. PCR on reverse transcribed RNA is a potent technique for the detection of IBV. In comparison with classical detection ...
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ژورنال
عنوان ژورنال: Fisheries science
سال: 1997
ISSN: 0919-9268
DOI: 10.2331/fishsci.63.592