Detecting nanometric displacements with optical ruler metrology

Optical Metrology Third Edition

Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores.

A technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.

Optical Metrology of Thin Films

A non-contact and non-destructive laser-based acoustic technique called impulsive stimulated thermal scattering (ISTS) is demonstrated for new applications in the context of a compact, portable apparatus. ISTS is used to determine viscoelastic and thermal transport properties of a DuPont Kapton film before and after neutron irradiation to determine the changes that would be expected to occur in...

Optical Metrology under Extreme Conditions

Optical metrology provides full field, noncontact, precise measurement of various physical parameters of materials, structures, and devices. These properties include kinematic parameters (displacement, velocity, and acceleration), deformation parameters (strains, curvature, and twist), surface parameters (shape and roughness), and mechanical properties of materials (Young’s modulus, Poisson’s r...

Optical Flow Estimation with Large Displacements: A Temporal Regularizer

The aim of this work is to propose a model for computing the optical flow in a sequence of images with a spatio–temporal regularizer explicitly designed for large displacements. We study the introduction of a temporal regularizer that expands the information beyond two consecutive frames. We propose to decouple the spatial and temporal regularizing terms to avoid an incongruous formulation betw...