Designing a less immunogenic nattokinase from Bacillus subtilis subsp. natto: a computational mutagenesis
نویسندگان
چکیده
منابع مشابه
In silico analysis of Nattokinase from Bacillus subtilis sp natto
Nattokinase or subtilisin NAT (EC 3.4.21.62) is one of the most remarkable enzymes produced by Bacillus subtilis sp. Natto, which posses direct fibrinolytic activity. The aim of this study is in silico analysis of Nattokinase structure and function. The three-dimensional structure of serine protease Nattokinase from Bacillus subtilis sp. natto was determined using homology modeling performed by...
متن کاملAmyloid-degrading ability of nattokinase from Bacillus subtilis natto.
More than 20 unrelated proteins can form amyloid fibrils in vivo which are related to various diseases, such as Alzheimer's disease, prion disease, and systematic amyloidosis. Amyloid fibrils are an ordered protein aggregate with a lamellar cross-beta structure. Enhancing amyloid clearance is one of the targets of the therapy of these amyloid-related diseases. Although there is debate on whethe...
متن کاملPurification and characterization of nattokinase from Bacillus subtilis natto B-12.
Bacillus subtilis natto B-12 was isolated from natto, a traditional fermented soybean food in Japan. A fibrinolytic enzyme (B-12 nattokinase) was purified from the supernatant of B. subtilis natto B-12 culture broth and showed strong fibrinolytic activity. The enzyme was homogenously purified to 56.1-fold, with a recovery of 43.2% of the initial activity. B-12 nattokinase was demonstrated to be...
متن کاملRelationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov.
Earlier phylogenetic studies based on the inferred DNA sequences of the polC, rpoB and gyrA genes suggested that strains of the species Bacillus subtilis formed two clusters, indicating the presence two closely related taxa; one contained the laboratory strain 168 and the other the laboratory strain W23. Significant sexual isolation was found between strain 168 and members of the group containi...
متن کاملComparative Fibrinolytic Activities of Nattokinases from Bacillus subtilis var. natto
Nattokinase is a potent fibrinolytic enzyme which was first found in a traditional Japanese soybean food. Two Nattokinase genes were cloned using chromosomal DNA from Bacillus subtilis (Ehrenberg 1835) Cohn 1872. Recombinant Natto-1.3 kb (GenBank accession number KF734090) and Natto-1.1 kb were expressed using a pQE-30 expression vector, and their fibrinolytic activities were studied. Natto-1.3...
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ژورنال
عنوان ژورنال: Journal of Molecular Modeling
سال: 2019
ISSN: 1610-2940,0948-5023
DOI: 10.1007/s00894-019-4225-y