Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
نویسندگان
چکیده
منابع مشابه
Diagnosis of infections with Leishmania infantum using PCR-ELISA.
On the basis of partial amplification of a cloned fragment of kDNA of Leishmania infantum which is specific for this species, we developed a PCR-ELISA technique which avoids the problems associated with classical diagnostic techniques. This technique was tested on 33 L. infantum strains from 19 different zymodemes, which were recognized equally. It was also used on human and canine clinical sam...
متن کاملA novel A2 allele found in Leishmania (Leishmania) infantum chagasi.
Visceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease is caused by Leishmania (Leishmania)infantum chagasi. Peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. The disease is endemic in Brazil and epidemic foci have been reported in densely populated cities all over the country. Many c...
متن کاملLeishmania (infantum) chagasi in canine urinary sediment.
Canine visceral leishmaniasis (CVL) is difficult to diagnosis, mainly due to the presence of asymptomatic animals, the diversity of clinical symptoms and the difficulty in obtaining diagnostic evidence of high sensitivity and specificity. The purpose of this study was to diagnose CVL in urinary sediment of 70 dogs of different breeds, sexes and ages from the veterinary hospital of the Federal U...
متن کاملFinal comments on an interesting taxonomic dilemma: Leishmania infantum versus Leishmania infantum chagasi.
It is grateful to see that the new session Readers opinion and discussion created after my comments Leishmania infantum versus Leishmania chagasi: do not forget the law of priority (Dantas-Torres 2006) on the concise review article of Lainson and Rangel (2005) has emerged as the basis of an interesting debate on the aetiological agent of American visceral leishmaniasis, i.e., the taxon...
متن کاملReproduction of Leishmania (Leishmania ) infantum chagasi in conditioned cell culture growth medium.
Leishmanias can be produced by inoculation in conditioned McCoy cell culture growth medium (CGM). Leishmania (Leishmania) infantum chagasi (100 parasites) grown in NNN medium was inoculated in 2.5 mL CGM, kept in plates (24 wells) and its multiplication was observed for five days (120 hours). After day 5, the medium was saturated with the flagellate forms of the parasite (promastigotes). The re...
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ژورنال
عنوان ژورنال: Revista Brasileira de Parasitologia Veterinária
سال: 2012
ISSN: 1984-2961
DOI: 10.1590/s1984-29612012000300024