Design of influenza diagnostic instrument based on biochemiluminescence

The design principle of a biochemiluminescent instrument is presented. diagnoses influenza based on detection the flu viral neuraminidase activity. uses photomultiplier tube (PMT) as sensor, with 24 test channels. reaction disk driven by step motor to rotate, where reagent incubated. Finally, PMT detects number photons emitted and calculates enzyme correlation coefficient (R2) 2.83 5.06 log TCID50/ml virus was 0.9967; limits two positive samples were TCID50/mL 953 TCID50/mL, respectively, predictive value (PPV) 100%, negative (NPV) 98.90% in clinical evaluation. According evaluation, designed can well match reagents, meet performance requirements setting.