Design of a Multi-Epitope Vaccine against Tuberculosis from Mycobacterium tuberculosis PE_PGRS49 and PE_PGRS56 Proteins by Reverse Vaccinology

Tuberculosis is a disease caused by Mycobacterium tuberculosis, representing the second leading cause of death an infectious agent worldwide. The available vaccine against this has insufficient coverage and variable efficacy, accounting for high number cases In fact, estimated third world’s population latent infection. Therefore, developing new vaccines crucial to preventing it. study, highly antigenic PE_PGRS49 PE_PGRS56 proteins were analyzed. These used predicting T- B-cell epitopes human leukocyte antigen (HLA) protein binding efficiency. Epitopes GGAGGNGSLSS, FAGAGGQGGLGG, GIGGGTQSATGLG (PE_PGRS49), GTGWNGGKGDTG (PE_PGRS56) selected based on their best physicochemical, antigenic, non-allergenic, non-toxic properties coupled HLA I II structures in silico assays. A construct with adjuvant (RS09) plus each epitope joined GPGPG linkers was designed, stability HLA-coupled further evaluated molecular dynamics simulations. Although experimental vivo studies are still necessary ensure its protective effect disease, study shows that dynamically stable potentially effective tuberculosis.