Design for large-scale maricultures of the Mediterranean demosponge Chondrosia reniformis Nardo, 1847 for collagen production

To support the use of marine demosponges for collagen production in integrated culture settings, we investigated a low–cost, easily applicable and sustainable method Chondrosia reniformis Nardo, 1847 ( Demospongiae , Chondrosiida Chondrosiidae ). Novel methods were tested to this collagen-rich demosponge three consecutive trials, during which recovery, survival growth rates explants monitored cultured within site with organic pollution (urban water discharge) pristine site. During Culture Trial 1, orientation plates had significant main effect on glued explants, vertically mounted showing faster compared horizontal ones (range 63 ± 46% 116 54%). However, detached more (30% versus 7%), was not case nailed where only 3% regardless orientation. Thus, yielded overall highest success rates. An interactive substrate attachment sponge also found: sponges growing PVC polypropylene (PP) as iron substrate, no differences found between substrates. 2, sites or found, although similar showed higher detachment (40–52%) lower Based results Trials 1 combination PP/nail/horizontal ultimately selected scale up 3, well colonies. 3 revealed 75–92% 83–100% colonies after 467 days culture. Growth at polluted both facing up/down 126 99% 218 139%) 128 60% 226 325%). These trials spanning 2 years consistent high over year culture, under conditions. We here report successful culturing pipeline using C. . The final ‘Demosponge lantern’ design, is simple, sustainable, enhances productivity adaptable seawater environments combined sources variable particle load such fish sewage outfall. • reniformis. study involves novel design that combines knowledge from our earlier studies information gained current study. termed as: ‘The Sponge lantern’. developed validated system provides secure delivers along