Design and utility of oligonucleotide gene probes for fungal polyketide synthases
نویسندگان
چکیده
منابع مشابه
Emergent gene order in a model of modular polyketide synthases.
Polyketides are a class of biologically active heteropolymers produced by assembly line-like multiprotein complexes of modular polyketide synthases (PKS). The polyketide product is encoded in the order of the PKS proteins in the assembly line, suggesting that polyketide diversity derives from combinatorial rearrangement of these PKS complexes. Remarkably, the order of PKS genes on the chromosom...
متن کاملAnalysis of the Sequences, Structures, and Functions of Product-Releasing Enzyme Domains in Fungal Polyketide Synthases
Product-releasing enzyme (PRE) domains in fungal non-reducing polyketide synthases (NR-PKSs) play a crucial role in catalysis and editing during polyketide biosynthesis, especially accelerating final biosynthetic reactions accompanied with product offloading. However, up to date, the systematic knowledge about PRE domains is deficient. In the present study, the relationships between sequences, ...
متن کاملThe stereochemistry of complex polyketide biosynthesis by modular polyketide synthases.
Polyketides are a diverse class of medically important natural products whose biosynthesis is catalysed by polyketide synthases (PKSs), in a fashion highly analogous to fatty acid biosynthesis. In modular PKSs, the polyketide chain is assembled by the successive condensation of activated carboxylic acid-derived units, where chain extension occurs with the intermediates remaining covalently boun...
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Microorganisms make a wealth of unusual metabolites that have a secondary role in the organism’s ontogeny, such as self-defense, aggression, or even communication as the need arises. These compounds often have biological activity valuable to humankind (1). Nevertheless, as valuable as antibiotics and other naturally occurring drugs have been over the past 60 years, their future use is threatene...
متن کاملDiversity-oriented combinatorial biosynthesis of benzenediol lactone scaffolds by subunit shuffling of fungal polyketide synthases.
Combinatorial biosynthesis aspires to exploit the promiscuity of microbial anabolic pathways to engineer the synthesis of new chemical entities. Fungal benzenediol lactone (BDL) polyketides are important pharmacophores with wide-ranging bioactivities, including heat shock response and immune system modulatory effects. Their biosynthesis on a pair of sequentially acting iterative polyketide synt...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2001
ISSN: 1074-5521
DOI: 10.1016/s1074-5521(00)90064-4