Design and synthesis of multifunctional polymeric micelles for targeted delivery in Helicobacter pylori infection

This study aimed to design a hyaluronic acid-based targeted, mucoadhesive and mucopenetrating drug delivery system encapsulating clarithromycin that would improve the residence time at H. pylori infection site. Clarithromycin-loaded thiolated acid-co-oleic acid (CLR-thHA-co-OA), clarithromycin-loaded ureido-conjugated (CLR-Ur-thHA-co-OA), papain-modified acid-co- oleic (CLR-PAP-Ur-thHA-co-OA) nanomicelles were prepared by an ultra-sonication method. FTIR data confirmed successful grafting of different ligands polymer backbone. XRD analysis revealed amorphous nature conjugate inside nanomicelles. The critical micelle concentration PAP-Ur-thHA-co-OA was found be 15.85 µg/ml. Also, sizes in range 210–258 nm. TEM CLR-PAP-Ur-thHA-co-OA their small size spherical shape. Zeta potential entrapment efficiency –24 mV 80 ± 7.2% respectively. A sustained release pattern observed for all types nanomicelles, whereas ones also displayed improved stability acidic pH. Due presence thiol groups backbone, important properties. In this regard, FDA loaded showed deepest penetration distance into mucus cleavage mucoglycoproteins when compared papain-unmodified Fluorescent images gastric tissues greater retention free drug. As consequence, enhanced vitro efficacy against vivo clearance eradication comparison other nanoformulations. Thus, newly synthesized have used as efficient nanocarrier treatment due mucopenetration mucoadhesion properties, well extended release.