Dental resin monomers induce early and potent oxidative damage on human odontoblast-like cells

Resin-based dental materials consist of filler particles and different monomers that are light cured in situ to re-establish function aesthetics. Due the degree conversion adhesive polymers, triethyleneglycol dimethacrylate (TEGDMA) 2-hydroxyethyl methacrylate (HEMA) released relatively high amounts susceptible degradation, acting as bioactive compounds affecting cell tissues. This study aimed assess effect HEMA TEGDMA exposure on metabolic activity, membrane integrity, survival human odontoblast-like (hOLCs). Exposure resin for 24 h induced major changes survival, which were measured by calcein method lactate dehydrogenase release. Increased early reactive oxygen species (ROS) production was observed leading degradative oxidation lipids identified malondialdehyde production. Severe alteration mitochondria occurred due transmembrane mitochondrial potential collapse, possibly inducing activation apoptotic death. hOLCs modified redox potential, with consequences permeability including function. Lipid peroxidation appears be a key phenomenon structures after exposure, death cytotoxicity. respond differential induction adaptive mechanisms maintain homeostasis. Modulation oxidative stress-induced response involves regulation genes encode antioxidant proteins such catalase heme oxygenase-1; functions critical protection mechanism against damage TEGDMA. Ascorbic acid an substance mitigates associated monomers.