Density, Distribution and Staining Properties of Eosinophilic Granular Cells in Oscar Fish (Astronotus ocellatus) Intestine

Fish eosinophilic granular cells are found in the connective tissues of structures associated with external environment. These similar to mammalian mast terms structural and functional characteristics. The cytoplasmic granules these give different staining reactions depending on fixative type. This study aimed determine properties densities Oscar fish (Astronotus ocellatus Agassiz, 1831) intestine using fixatives histochemical techniques. Formalin basic lead acetate fixation-giemsa indicated that were abundant anterior intestine, localizing around especially blood vessels submucosa. Giemsa Bouin’s fixed rather than other showed higher posterior intestine. No reaction was observed Thionin Toluidine Blue any fixative. Eosinophilic samples had metachromatic Alcian staining. In conclusion, this shows may have effects distribution intestinal regions. Abundance submucosal could play an active role mucosal immunity against food-borne pathogens.