DEMONSTRATION OF STAPHYLOCOCCAL CLUMPING FACTOR AND FREE COAGULASE IN SOFT AGAR MEDIA
نویسندگان
چکیده
منابع مشابه
Demonstration of staphylococcal clumping factor and free coagulase in soft agar media.
Of the methods now available for the detection of coagulase positive staphylococei, none is designed to show both clumping factor and free coagulase activity in a single system. The observations reported here are by-products of a search for variant strains of staphylococci with particular reference to strain differences in the cell surface component known as clumping factor or bound coagulase. ...
متن کاملFactors affecting the demonstration of staphylococcal free coagulase and clumping factor.
With the use of Phenol Red Mannitol (PRM) Broth (Difco) as a basal medium, rather than Trypticase Soy (TS) Broth (BBL), we were able to modify a previously described plasma-fibrinogen-soft agar medium (S. Y. Alami and F. C. Kelly, J. Bacteriol. 78:539, 1959) for the simultaneous demonstration of mannitol fermentation (MF), free coagulase (FC), and clumping factor (CF) production by staphylococc...
متن کاملCharacteristics of coagulase positive and coagulase negative staphylococci in serum-soft agar.
Preliminary experiments revealed that coagulase positive staphylococci could be distinguished culturallv from coagulase negative staphylococci in a soft agar medium containing a small amount of normal human or rabbit plasma. In a medium of suitably low viscosity, staphylococci produce elongated diffuse feathery colonies. When coagulable plasma is incorporated in such a medium, the colonies of c...
متن کاملComparison of staphylococcal clumping factor and protein A.
Protein A (3, 8) and the clumping factor (bound coagulase; 1) of Staphylococcus aureus share common characteristics, yet, to our knowledge, they have not been compared to determine possible identity. Both are protein, antigenic, cell surface components of S. aureus but not of S. epidermidis, and both are released into the culture medium as growth develops. Protein A is a common agglutinogen of ...
متن کاملPurification of staphylococcal coagulase.
Correlation between the pathogenicity of certain staphylococci and their ability to produce coagulase has led to extensive studies of the clotting enzyme. Walston (1935) precipitated the clotting substance from cell-free filtrates of appropriate staphylococcal cultures by the use of ethanol, acetic acid, or ammonium sulfate. Walker et al. (1948) partially purified staphylocoagulase by repeated ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1959
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.78.4.539-544.1959