Demonstration of iNOS-mRNAand iNOS in human monocytes stimulated with cancer cells in vitro
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Demonstration of iNOS-mRNA and iNOS in human monocytes stimulated with cancer cells in vitro.
Synthesis and localization of inducible nitric oxide synthase mRNA (iNOS-mRNA) and iNOS protein in the cultures of human monocytes (Mphi) and colon carcinoma cell line (DeTa) that resulted in nitric oxide (NO) synthesis has been studied. The iNOS-mRNA was observed around the sixth hour of culture and peaked at the twelfth hour. The iNOS-mRNA, as determined by the in situ hybridization and iNOS ...
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Inducible nitric oxide synthase (iNOS) gene expresses a calcium calmudolin-independent enzyme which can catalyse NO production from L-arginine. The induction of iNOS activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (LPS). Previous studies indicated that all nitric oxide synthases (NOS) activated in human umbilical vein endot...
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inducible nitric oxide synthase (inos) gene expresses a calcium calmudolin-independent enzyme which can catalyse no production from l-arginine. the induction of inos activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (lps). previous studies indicated that all nitric oxide synthases (nos) activated in human umbilical vein endot...
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Background: Microglial cells act as the sentinel of the central nervous system .They are involved in neuroprotection but are highly implicated in neurodegeneration of the aging brain. When over-activated, microglia release pro-inflammatory factors, such as nitric oxide (NO) and cytokines, which are critical in eliciting neuroinflammatory responses associated with neurodegenerative diseases. Thi...
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ژورنال
عنوان ژورنال: Journal of Leukocyte Biology
سال: 1999
ISSN: 0741-5400
DOI: 10.1002/jlb.65.5.597