منابع مشابه
Defining piRNA primary transcripts.
PIWI proteins and their associated small RNAs (PIWI-interacting RNAs, piRNAs) are essential for fertility in mammals. piRNAs (24–35 nt) are longer than miRNAs (21–23 nt) and have 2'-O-methyl-modified 3' termini. Like miRNAs, piRNAs bind members of the Argonaute family of proteins, but piRNAs are unique in that they guide PIWI proteins, a specialized subfamily of Argonaute proteins that are expr...
متن کاملpiRNA-directed cleavage of meiotic transcripts regulates spermatogenesis.
MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. To identify meiotic piRNA targets, we augmented the mouse piRNA repertoire by introducing a human meiotic piRNA cluster. This triggered a spermatogenesis defect by inappropriately targeting the piRNA machinery to mouse mRNAs essential for germ cell development. A...
متن کاملMinotaur is critical for primary piRNA biogenesis.
Piwi proteins and their associated small RNAs are essential for fertility in animals. In part, this is due to their roles in guarding germ cell genomes against the activity of mobile genetic elements. piRNA populations direct Piwi proteins to silence transposon targets and, as such, form a molecular code that discriminates transposons from endogenous genes. Information ultimately carried by piR...
متن کاملPrimary piRNA biogenesis: caught up in a Maelstrom.
Precursors for most Piwi-interacting RNAs (piRNAs) are indistinguishable from other RNA polymerase II-transcribed long noncoding RNAs. So, it is currently unclear how they are recognized as substrates by the piRNA processing machinery that resides in cytoplasmic granules called nuage. In this issue, Castaneda et al (2014) reveal a role for the nuage component and nucleo-cytoplasmic shuttling pr...
متن کاملRNA Clamping by Vasa Assembles a piRNA Amplifier Complex on Transposon Transcripts
Germline-specific Piwi-interacting RNAs (piRNAs) protect animal genomes against transposons and are essential for fertility. piRNAs targeting active transposons are amplified by the ping-pong cycle, which couples Piwi endonucleolytic slicing of target RNAs to biogenesis of new piRNAs. Here, we describe the identification of a transient Amplifier complex that mediates biogenesis of secondary piR...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2013
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.24989