Defining human dendritic cell progenitors by multiparametric flow cytometry
نویسندگان
چکیده
منابع مشابه
Multiparametric analysis of apoptosis by flow and image cytometry.
Flow cytometric assays for apoptosis are now in widespread use. The multiparametric nature of flow cytometry allows multiple assays for several apoptotic characteristics to be combined in a single sample, providing a powerful tool for elucidating the complex progression of apoptotic death in a variety of cell types. This chapter describes one such assay, allowing simultaneous analysis of caspas...
متن کاملDetection of microparticles from human red blood cells by multiparametric flow cytometry.
BACKGROUND During storage, red blood cells (RBC) undergo chemical and biochemical changes referred to as "storage lesions". These events determine the loss of RBC integrity, resulting in lysis and release of microparticles. There is growing evidence of the clinical importance of microparticles and their role in blood transfusion-related side effects and pathogen transmission. Flow cytometry is ...
متن کاملProliferating dendritic cell progenitors in human blood
CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. CD34+ cells are rare in adult blood, however, making it difficult to use CD34+ cells to ascertain if DC progenitors are present in the circulation and if blood can be a starting point to obtain large numbers of these immunostimulatory antigen-presenting cells for clin...
متن کاملAutomation of human sperm cell analysis by flow cytometry.
Semen sample analysis is routinely performed by microscopical evaluation and manual techniques by laboratory operators; the analysis is affected by a wide imprecision related to variability among observers, influencing its clinical validity. Our aim was to automate sperm analysis with the use of flow cytometry for evaluation of cell counts and typing and with the use of a new membrane-permeant ...
متن کاملMultiparametric Analysis of Aquatic Organisms Using Flow Cytometry
Environmental research on aquatic microorganisms, such as phytoplankton, aquatic bacteria, and aquatic viruses, often takes advantage of their natural chlorophyll, phycobilins, and other intrinsic fluorescent pigments. Although epifluorescence microscopy is still used frequently, flow cytometry has become a key research tool due to its ability to provide multiparametric analysis at the single-c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Protocols
سال: 2015
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2015.092