Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores
نویسندگان
چکیده
منابع مشابه
Directed evolution of CotA laccase for increased substrate specificity using Bacillus subtilis spores.
Directed evolution is an effective strategy to engineer and optimize protein properties, and microbial cell-surface display is a successful method to screen protein libraries. Protein surface display on Bacillus subtilis spores is demonstrated as a tool for screening protein libraries for the first time. Spore display offers advantages over more commonly utilized microbe cell-surface display sy...
متن کاملSurface display of recombinant proteins on Bacillus subtilis spores.
We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression ...
متن کاملCotA of Bacillus subtilis is a copper-dependent laccase.
The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA nor other sporulation proteins are involved. We demonstrate that CotA is a laccase. Syringaldazine, a specific substrate of laccases, reacted with wild-type spores but not with DeltacotA spores. Co...
متن کاملExpression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores
BACKGROUND The bacterial endospore (spore) has recently been proposed as a new surface display system. Antigens and enzymes have been successfully exposed on the surface layers of the Bacillus subtilis spore, but only in a few cases the efficiency of expression and the effective surface display and have been determined. We used this heterologous expression system to produce the A subunit of the...
متن کاملSurface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores
Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determine...
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ژورنال
عنوان ژورنال: Journal of Microbiology and Biotechnology
سال: 2019
ISSN: 1017-7825,1738-8872
DOI: 10.4014/jmb.1907.07019