Decellularized pericardium tissues at increasing glucose, galactose and ribose concentrations and at different time points studied using scanning X-ray microscopy

Diseases like widespread diabetes or rare galactosemia may lead to high sugar concentrations in the human body, thereby promoting formation of glycoconjugates. Glycation collagen, i.e. glucose bridges, is nonenzymatic and therefore cannot be prevented any other way than keeping level low. It relates secondary diseases, abundantly occurring aging populations diabetics. However, little known about effects glycation collagen on molecular level. We studied vitro effect glycation, with D-glucose D-galactose as well D-ribose, structure type 1 by preparing decellularized matrices bovine pericardia soaked different solutions, at increasing (0, 2.5, 5, 10, 20 40 mg ml −1 ), incubated 37°C for 3, 14, 30 90 days. The tissue samples were analyzed small- wide-angle X-ray scattering scanning mode. found that galactose produce similar changes they mainly affect lateral packing between macromolecules. ribose much faster provoking a larger packing, but also seems cause qualitatively structure.