Decellularization of Human Umbilical Arteries
نویسندگان
چکیده
منابع مشابه
Constriction of Human Umbilical Arteries
Experiments on perfused segments or isolated rings of human umbilical artery showed that the magnitude of their contraction in response to bradykinin, epinephrine, or serotonin depended on the P02 of the perfusing or bathing solution. Constriction to bradykinin varied directly with the O2 tension and was greatest when the Po2 was approximately that of umbilical arterial blood during the transit...
متن کاملProteoglycans of human umbilical cord arteries.
Proteoglycans (PGs) were dissociatively extracted from human umbilical cord arteries (UCAs) with 4 M guanidine hydrochloride containing Triton X-100 and protease inhibitors, purified by Q-Sepharose anion exchange chromatography and lyophilized. They were analysed by gel filtration, SDS/PAGE and agarose gel electrophoresis before and after treatment with chondroitinase ABC. It was found that the...
متن کاملSmall vessel replacement by human umbilical arteries with polyelectrolyte film-treated arteries: in vivo behavior.
OBJECTIVE The aim of this study was to evaluate the patency of human umbilical arteries treated with polyelectrolyte multilayers (PEMs) after rabbit implantation. BACKGROUND The development of small-caliber vascular substitutes with high patency after implantation remains a real challenge for vascular tissue engineering. METHODS Cryopreserved human umbilical arteries were enzymatically de-e...
متن کاملIntimal ultrastructure of human umbilical arteries. Observations on arteries from newborn children of smoking and nonsmoking mothers.
The umbilical artery was chosen as a possible model for evaluating the vascular injury provoked by tobacco smoking in humans. Cords from newborn children delivered by 15 nonsmoking and 13 smoking mothers were studied in the transmission and the scanning electron microscope. Pronounced intimal changes were seen in the arteries from smoking mothers; the most important findings were degenerative c...
متن کاملUtilization of Pulsatile flow to Decellularize the Human Umbilical Arteries to Make Small-Caliber Blood Vessel Scaffolds.
PURPOSE To explore the effect of pulsatile flow in the decellularization process of small blood vessels. METHODS A total of 30 human umbilical cords were used in the current study. The umbilical cords were flushed with 0.25% trypsin/0.01% EDTA for 30 minutes, followed by treatment with 1% sodium dodecyl sulfate sodium dodecyl sulfate for 3 hours. The effectiveness of decellularization on the ...
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ژورنال
عنوان ژورنال: International Journal of Morphology
سال: 2019
ISSN: 0717-9502
DOI: 10.4067/s0717-95022019000100111