De novo Transcriptome Assembly and Comprehensive Annotation of Two Tree Tomato Cultivars (Solanum betaceum Cav.) with Different Fruit Color

The tree tomato (Solanum betaceum Cav.) is an underutilized fruit crop native to the Andean region and phylogenetically related potato. Tree fruits have a high amount of nutrients bioactive compounds. However, so far there are no studies at genome or transcriptome level for this species. We performed de novo assembly annotation purple-fruited (A21) orange-fruited (A23) accessions. A total 174,252 194,417 transcripts were assembled with average length 851 849 bp. 34,636 36,224 showed significant similarity known proteins. Among annotated unigenes, 22,096 23,095 assigned Gene Ontology (GO) term 14,035 14,540 found Clusters Orthologous Group (COG) classifications. Furthermore, 155 161 KEGG pathways. carotenoid biosynthetic process GO terms significantly enriched in accession A21. Finally, 68,647 intraspecific single-nucleotide variations (SNVs) almost 2 million interspecific SNVs identified. results study provide wealth genomic data genetic improvement tomato.