De novo QTL-seq Identifies Loci Linked to Blanchability in Peanut (Arachis hypogaea) and Refines Previously Identified QTL with Low Coverage Sequence

Blanchability is an often overlooked, but important trait for peanut breeding. The process of blanching, removing the skin, step in processing raw nuts manufacturing. Under strong genetic control and requiring considerable effort to phenotype, blanchability conducive marker-assisted selection. We used QTL sequencing (QTL-seq) identify two QTLs related using previously phenotyped breeding populations. To validate QTLs, we show that markers can select significantly increased independent recombinant inbred line (RIL) population. Two wild introgressions from Arachis cardenasii conferring disease resistance were segregated population found negatively impact blanchability. Finally, by utilizing highly accurate sequence analysis pipelines, low coverage be genotype whole populations with power precision. This study highlights potential mine data develop useful improvement programs, provide powerful tools quality traits.