Dark-Field Electron Microscopy of Thin Sections of Trichosporon cutaneum

Trichosporon cutaneum C.A.P.D. peritonitis.

Electron microscopy of ultra-thin sections of lepromatous peripheral nerves.

The submicroscopic structure of peripheral nerves are now being revealed by many investigators (1 , 2, 3, 8) using the high resolving power of the electron microscope. Stimulated by this recent progress in neurology, we have made an electron microscope study of ultra-thin sections of leprous peripheral nerves. In the present article we report on such a study of sections of great auricular nerve...

Uptake of phenol by Trichosporon cutaneum.

The soil yeast Trichosporon cutaneum, which is distinguished by having a strictly oxidative metabolism, can be induced to utilize phenol as a sole carbon source. The present paper shows that such phenol-induced cells contain a specific, energy-dependent uptake system for phenol. Phenol uptake is not directly linked to its o-hydroxylation inside the cell, the first step of phenol metabolism. The...

Visualization of nucleosomes in thin sections by stereo electron microscopy

Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stabil...

cis,cis-Muconate cyclase from Trichosporon cutaneum.

The inducible enzyme catalysing the conversion of cis,cis-muconate to (+)-muconolactone was purified 300-fold from the yeast Trichosporon cutaneum, grown on phenol. The enzyme has a sharp pH optimum at pH 6.6. It reacts also with several monohalogen derivatives and with one monomethyl derivative of cis,cis-muconate, but not with cis,trans- or trans,trans-muconate or 3-carboxy-cis,cis-muconate. ...