Cytotoxicity assessment of Aflatoxin B1 after high voltage atmospheric cold plasma treatment

Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus, known carcinogen in humans animals. High voltage atmospheric cold plasma (HVACP) technology has already shown promise to decontaminate AFB1 food feed. This study aimed investigate the cytotoxicity of after HVACP treatment. (100 ?M) was treated at 85 kV with for 0, 2, 5, 10, 20 min. HepG2 cells were exposed HVACP-treated 72 h assessed cell viability, caspase-3 activity, DNA fragmentation, protein carbonyls each treatment time. Cell fragmentation levels, contents min not significantly different compared non-exposed (P > 0.05). However, their higher other times < 0.01). Twenty minutes reduced oxidative damage showed potential as safe aflatoxin decontamination technology.