Cytotoxic and Anti-proliferative Effects of Moringa oleifera Lam. on HeLa Cells
نویسندگان
چکیده
Background: The aim of the study was to determine mechanism Moringa oleifera-induced apoptosis in HeLa cells. cells over-express cyclin E and B1, abrogate G0-G1 G2-M cell cycle arrest, promoting tumorigenesis. Cyclin E, E2F1 telomerase expression, caspase-3 -7 activation were assessed after 24-treatment with M. oleifera leaf fractions. Material methods: Apoptosis through caspase-7 determined quantitatively by FAM FLICA™ Caspase-3/7 assay. B1 quantified flow cytometry. Telomerase evaluated Telomeric repeat amplification protocol (TRAP reaction). effects on colony formation seeding treated six-well plates for 7 days under culture conditions. MTT assay used survival. Results: 24 hours fractions showed dose-dependent cytotoxicity, caspases-3 -7, down regulation E2F1, inhibition expression. Cell analysis dead population cellcycle arrest. Conclusion: triggered mitochondrial pathway arrest at phase 24-hour treatment, activation. In addition, extract induces senescence down-regulation telomerase. Colony proliferation inhibited a manner, corresponding inhibition.
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ژورنال
عنوان ژورنال: The Natural products journal
سال: 2023
ISSN: ['2210-3155', '2210-3163']
DOI: https://doi.org/10.2174/2210315511666210826165242