Cytoplasmic Dynein LC8 Interacts with Lyssavirus Phosphoprotein
نویسندگان
چکیده
منابع مشابه
Cytoplasmic dynein LC8 interacts with lyssavirus phosphoprotein.
Using a yeast two-hybrid human brain cDNA library screen, the cytoplasmic dynein light chain (LC8), a 10-kDa protein, was found to interact strongly with the phosphoprotein (P) of two lyssaviruses: rabies virus (genotype 1) and Mokola virus (genotype 3). The high degree of sequence divergence between these P proteins (only 46% amino acid identity) favors the hypothesis that this interaction is ...
متن کاملEbolavirus VP35 interacts with the cytoplasmic dynein light chain 8.
The viral protein VP35 of ebolavirus (EBOV) is implicated to have diverse roles in the viral life cycle. We employed a yeast two-hybrid screen to search for VP35 binding partners and identified the cytoplasmic dynein light chain (DLC8) as a protein that interacts with VP35. Mapping analysis unraveled a consensus motif, SQTQT, within VP35 through which VP35 binds to DLC8. The disruption of DLC8 ...
متن کاملInteractions of LC8 with N-terminal segments of the intermediate chain of cytoplasmic dynein.
LC8, a highly conserved 10-kDa light chain, and IC74, a 74-kDa intermediate chain, are presumed to promote the assembly of the cytoplasmic dynein motor protein complex and to be engaged in the controlled binding and release of cargo. The interactions of LC8 from Drosophila melanogaster with constructs of IC74 were characterized in vitro by affinity methods, limited proteolysis, and circular dic...
متن کاملInteractions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74.
The interactions of three subunits of cytoplasmic dynein from Drosophila melanogaster, LC8, Tctex-1, and the N-terminal domain of IC74 (N-IC74, residues 1-289), were characterized in vitro by affinity methods, limited proteolysis, and circular dichroism spectroscopy. These subunits were chosen for study because they are presumed to promote the assembly of the complex and to be engaged in the co...
متن کاملhev-orf۳ encoding phosphoprotein interacts with hepsin
conclusions out of eight screened proteins interacting with porf3, hepsin was confirmed to have specific interactions with porf3. results through the cyto-trap two-hybrid system, eight proteins interacting with porf3 were winnowed. the co-ip results confirmed that hepsin which is reported to function as the inhibitor of several tumors reacted with porf3. materials and methods the cyto-trap two-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 2000
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.74.21.10217-10222.2000