Cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase from the hamster. II. Isolation of the gene and characterization of the 5' flanking region.
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چکیده
منابع مشابه
Cytoplasmic 3-Hydroxy-3-methylglutaryl Coenzyme A Synthase from Liver
The cytoplasmic fraction of avian liver contains multiple forms of 3-hydroxyd-methylglutaryl coenzyme A synthase which are distinct from their mitochondrial counterpart. Of the four cytoplasmic species, synthases I and II predominate, constituting 22 and 62%, respectively, of the total cytoplasmic synthase activity; synthases III and IV are minor forms comprising the remaining 16%. Each of the ...
متن کاملLocalization of the gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A synthase to human chromosome 5.
A series of hybrids between primary human cells and a Chinese hamster somatic cell mutant (Mev-1), defective in expression of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase [(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating, EC 4.1.3.5], has been prepared that complements the mutant defect. A technique based on differential sensitivity of this enzyme...
متن کاملMultiple sterol regulatory elements in promoter for hamster 3-hydroxy-3-methylglutaryl-coenzyme A synthase.
Through substitution mutagenesis and gene transfer experiments in cultured cells, we have identified three sequences in the 5' flanking region of the gene for hamster 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase that are required for sterol-mediated regulation of transcription. Point mutations in any one of these sequences largely prevented the increase in transcription that normall...
متن کاملClass II 3-hydroxy-3-methylglutaryl coenzyme A reductases.
The biosynthesis of isopentenyl diphosphate (isopentenyl pyrophosphate), the precursor of isoprenoids in all forms of life, occurs by two distinct metabolic pathways, the mevalonate pathway (Fig. 1) and the glyceraldehyde 3-phosphate/pyruvate pathway, often termed the nonmevalonate pathway (17). Whereas many gram-negative bacteria employ the nonmevalonate pathway (26), humans, other eukaryotes,...
متن کاملIsolation and characterization of a mammalian cell mutant defective in 3-hydroxy-3-methylglutaryl coenzyme A synthase.
A somatic cell mutant of the Chinese hamster ovary (CHO)-K1 cell auxotrophic for mevalonic acid has been isolated by means of the bromodeoxyuridine-visible light technique. This mutant can incorporate labeled mevalonate but not labeled acetate into cholesterol and, thus, is apparently defective in mevalonate biosynthesis. The mutant is recessive with respect to the parental cell phenotype. Asse...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)35706-x