منابع مشابه
Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase.
Two mutants with specific defects in cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1) have been isolated from cultures of Saccharomyces cerevisiae exposed to the mutagens ethyl-methane sulfonate and Mn++. The mutations have been shown to be extranuclear by two criteria. The phenotype persists in diploids formed by a cross with a p-o strain of yeast of the opposite mati...
متن کاملCytochrome c(3) mutants of Desulfovibrio desulfuricans.
To explore the physiological role of tetraheme cytochrome c(3) in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated plasmid showed that the insertion was successful. The growth rate of the mutant on lactate with sulfa...
متن کاملMutants of Yeast Deficient in Cytochrome C.
YTOCHROME C is an ideal protein for biochemical studies because of its ease of isolation and low molecular weight. Its primary structure from a number of species is entirely (cf. SMITH, MATSUBARA, MCDOWALL and ROTHFUS 1963) or partially (cf. TUPPY 1958) known. Therefore, any system exhibiting genetic control of cytochrome c would be of considerable interest for investigating geneprotein relatio...
متن کاملParacoccus denitrificans cytochrome c1 gene replacement mutants.
We describe the construction and characterization of gene replacement mutants for the respiratory chain component cytochrome c1 in the bacterium Paracoccus denitrificans. Its structural gene (fbcC) was inactivated by insertion of the kanamycin resistance gene, introduced into a suicide vector, and conjugated into Paracoccus; chromosomal mutants obtained by homologous recombination were selected...
متن کاملAnalysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast.
Cytochrome oxidase catalyses the reduction of oxygen to water. The mitochondrial enzyme contains up to 13 subunits, 11 in yeast, of which three, Cox1p, Cox2p and Cox3p, are mitochondrially encoded. The assembly pathway of this complex is still poorly understood. Its study in yeast has been so far impeded by the rapid turnover of unassembled subunits of the enzyme. In the present study, immunobl...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1969
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2036