منابع مشابه
Cytochemical methods of hormone assay LUCILLE
Initially polypeptide hormones were assayed by their biological activity. Such conventional bioassays, however, are remarkably insensitive. For example, the Lipscomb and Nelson (1962) bioassay of corticotrophin (ACTH) cannot measure a concentration of less than about 100 pg/ml, while the normal circulating levels of the bioactive form of this hormone range from about 5 to 60 pg/ml. The introduc...
متن کاملCytochemical methods of hormone assay LUCILLE BITENSKY
Initially polypeptide hormones were assayed by their biological activity. Such conventional bioassays, however, are remarkably insensitive. For example, the Lipscomb and Nelson (1962) bioassay of corticotrophin (ACTH) cannot measure a concentration of less than about 100 pg/ml, while the normal circulating levels of the bioactive form of this hormone range from about 5 to 60 pg/ml. The introduc...
متن کاملCytochemical bioassay of parathyroid hormone: characteristics of the assay and analysis of circulating hormonal forms.
A cytochemical bioassay for parathyroid hormone (PTH) was used for the characterization of the biological activity of circulating forms of the hormone. PTH-stimulated glucose-6-phosphate dehydrogenase activity in distal convoluted tubule cells was quantitated by integrating microdensitometry and the response to native bovine (b)PTH(1-84) was found to be linear between graded doses of hormone fr...
متن کاملParathyrin (parathyroid hormone): metabolism and methods for assay.
Parathyrin (parathyroid hormone) radioimmunoassay is a commonly ordered endocrine assay. I discuss here of the metabolism of the hormone and the effect information on this metabolism has had on the development of radioimmunoassays for parathyrin. Although radioimmunoassay is the most widely accepted technique for the routine assay for parathyrin, "high-performance" liquid chromatography, cytoch...
متن کاملMethods in Determining Growth Hormone Concentrations : An Immunofunctional Assay
Actual measurement of growth hormone (GH) levels in children suspected of having GH deficiency can confirm this diagnosis. However, there currently is no standard for the test. Although there is general agreement that in a short-statured child, a value of <10 mg/L in response to an appropriate provocative test indicates the possible need for GH replacement therapy, there is no agreement on the ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1976
ISSN: 0021-9746
DOI: 10.1136/jcp.s1-7.1.22