Cyto-histological aspects of haploid androgenesis when obtaining haploids/doubled haploids in rice (Oryza Sativa l.) anther culture <i>in vitro</i>

The aim of the study was to development rice microspores in anther culture vitro , structure androclinic callus identify cyto-embryological features formation morphogenic structures culture, obtain doubled haploids ( Oryza sativa L .) and accelerate valuable breeding material with desired properties. Within framework this study, results a cyto-histological haploid androgenesis were obtained, which indicate that it is induced under influence phytohormones from at mononuclear or early binuclear stage development. abnormal on nutrient media phytohormone 2,4-D traced, nuclei, having lost their characteristic functions, acquired ability unlimited division growth microcallus. morphological calli assessed. main morphotypes tissues pathways morphogenesis, leading androgenic structures, up full-fledged regenerant plants, identified. Homozygous lines based F 1 BC - generations obtained course hybridization backcrossing between Chinese samples carrying blast resistance genes Russian accessiond rapidly developed.