Cyclic Hexapeptide from Bouvardia ternifolia (Cav.) Schltdl. and Neuroprotective Effects of Root Extracts

Bouvardia ternifolia (Cav.) Schltdl. is a shrub that belongs to the Rubiaceae family and distributed throughout México; it has been used for its antioxidant, neuroprotective, anti-inflammatory properties. This work aimed evaluate protective effects of B. root extracts on blood-brain barrier positive regulation cytokines IL-1β, IL-6, TNF-α, characterization compounds present in dichloromethane (BtD) hexane (BtH) extracts. Male ICR mice were orally administered with 5 days before single injection LPS. Administration BtH BtD significantly decreased Evans blue leakage into brain tissue by 70% 68%, respectively. Meloxicam (MX) concentration IL-1β 39.6%; BtM 53.9%; BtAq 48.4%; 31.9%, 37.7%. was only treatment IL-6 32.2%. The TNF-α declined each treatments. chemical composition characterized GC–MS, cyclic hexapeptide identified 13C, 1H NMR, two-dimension techniques. In extract, seven found 13 found. methanolic (BtM) aqueous (BtAq) not subjected analysis, because they did show significant difference BBB protection activity. Therefore, results suggested protect barrier, maintaining stable selective permeability, thereby preventing LPS from entering tissue. Simultaneously, modulate production TNF-α. It important note this research evaluated complete