Cyclic adenosine 3’5’monophosphate (cAMP) regulates meiotic maturation in goldfish oocytes Carassius auratus (Teleostei: Cyprinidae)

A good understanding of the mechanism oocyte development and maturation, regulatory factors involved therein are important for success a breeding program especially species used in aquaculture. It is crucial females to supply high quality mature oocytes, which generally achieved by fine-tuning meiotic arrest resumption progression. Therefore, knowledge maturation process fundamental methodologies increase fish production TheFigure regulations still not totally understood. The hypothesis whether elevated levels or drop intraoocyte cAMP triggers was investigated denuded goldfish oocytes. In presence agents that elevate like forskolin, dibutyryl cAMP, isobutyl methylxanthine, oocytes inhibited. Whereas 2’deoxyadenosine-3-monophosphate, an inhibitor adenylate cyclase promoted maturation. Forskolin decreased spontaneous as well hormone-induced At 10 µM forskolin added up 4 h after hormonal stimulation completely inhibited Beyond h, however, when had already started, can no longer inhibit Intraoocyte assayed with without immediately within 30 minutes. With it did cause significant decrease until at h. results were consistent findings maintain while necessary sufficient trigger