Current status and molecular phylogeny of some economically important viruses infecting wheat crops of Şırnak province, Turkey

A survey of yellow dwarf viruses (YDVs) -associated diseases wheat plants was performed for the first time in Şırnak Province, Turkey. In cultivated areas, symptoms such as diminished leaf size, dwarfing, yellowing, and reddening leaves were observed areas. total 441 specimens collected regardless showing assayed Barley/Cereal Yellow Dwarf Viruses [B/CYDV] association by PCR tests using virus coat protein gene (CPG)-specific primer pairs. These assays demonstrated that 13.38% (55 samples) positive to occurrence BYDV-PAV (9.73%) CYDV-RPV (0.73%) viruses, but no BYDV-MAV, -SGV, -RMV infections BYDVs found samples. addition, double infection detected 12 samples with a 2.91% rate. The CPG sequences randomly selected two isolates revealed bacterial cloning sequencing. Sequences obtained deposited GenBank under Accession numbers OL685734 OL685736 BYDV-PAV; OL685735 OL685737 CYDV-RPV. BLASTn analysis four shared high nucleotide homology their intraspecies isolates, 97.35-98.67% 97.72-97.89 consensus tree constructed classified B/CYDV-associated phylogenetically closest individuals from Turkey world. This is report associated Province Keywords: BYDVs; Coat gene; Incidence; Sequencing;