Culturing human umbilical cord blood: a comparison of mononuclear vs CD34+ selected cells
نویسندگان
چکیده
منابع مشابه
CD34+ stem cells from umbilical cord blood
We describe the relation between umbilical cord clamping time and two different enrichment system of CD34(+) stem cells from umbilical cord blood with the proliferative ability and bone marrow reconstitution of the stem cells obtained. After an obstetrician performed the cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negat...
متن کاملTherapeutic Benefit of Intravenous Administration of Human Umbilical Cord Blood- Mononuclear Cells Following Intracerebral Hemorrhage in Rat
Objective(s) Human umbilical cord blood (HUCB) is now considered as a valuable source for stem cell–based therapies. Previous studies showed that intravascular injection of the HUCB significantly improves neurological functional recovery in a rat model of intracerebral hemorrhage (ICH). In the present study, we hypothesize transplanted HUCB derived mononuclear cells (UC-MCs) can decrease injur...
متن کاملIntra-osseous Co-transplantation of CD34-selected Umbilical Cord Blood and Mesenchymal Stromal Cells
Human mesenchymal stromal cells (MSC) have been shown to support the growth and differentiation of hematopoietic stem cells (HSC). We hypothesized that intra-osseous (IO) co-transplantation of MSC and umbilical cord blood (UCB) may be effective in improving early HSC engraftment, as IO transplantation has been demonstrated to enhance UCB engraftment in NOD SCID-gamma (NSG) mice. Following non-l...
متن کاملCorrelation of umbilical cord blood volume with CD34+ cells concentration
Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematopoitic progenitor cells for clinical application. The parameters commonly used to evaluate a UCB unit and predict transplant outcomes have been total nucleated cell count (TNCs), CD34+ cells concentration and total volume of cord blood collected. The volume of cord blood collection is also important ...
متن کاملCD34 enrichment of umbilical cord blood.
OBJECTIVE We describe the relation between the clamping time and blood volume collected, plus two enrichment systems for CD34+ stem cells from umbilical cord blood, to achieve an excellent recovery of cells with a high proliferative ability and bone marrow reconstitution. METHOD After cord blood collection by an obstetrician, stem cell purification has been performed either with a combination...
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ژورنال
عنوان ژورنال: Bone Marrow Transplantation
سال: 1999
ISSN: 0268-3369,1476-5365
DOI: 10.1038/sj.bmt.1701776