Cultivation and Serial Transfer of the Slime Mould, Dictyostelium discoideum in Liquid Nutrient Medium
نویسندگان
چکیده
منابع مشابه
Analysis of optical density wave propagation and cell movement in the cellular slime mould Dictyostelium discoideum
We have studied optical density wave propagation during aggregation of the cellular slime mould Dictyostelium discoideum in a quantitative manner by digital image analysis. The waves arc mostly single ended spiral waves starting from an aggregation center. We can measure a variety of parameters such as oscillation frequency, wave propagation velocity and wave shape. This allows the construction...
متن کاملStationary phase and the cell cycle of Dictyostelium discoideum in liquid nutrient medium.
Cells of the axenic strain of the cellular slime mould Dictyostelium discoideum, AX-3, multiply in the liquid nutrient medium HL-5 with a doubling time of 12 h. When the cell concentration reaches approximately 1 X10(7) per ml the rate of cell multiplication begins decreasing and after 20-30 h reaches zero, at a stationary phase cell concentration of 2 to 2-5 X 10(7) cells per ml. The intercept...
متن کاملExperimentally induced aberrations in the pattern of differentiation in the cellular slime mould Dictyostelium discoideum.
This paper describes a set of perturbatory experiments designed to elucidate aspects of the mechanism by which the normal pattern of differentiation is specified. Experiments are described which investigate the alterations in development seen in aqueous environments, with changes in humidity and with the introduction of permeable and impermeable barriers. The following results are reported: (i)...
متن کاملGrowth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملTwo dephosphorylation pathways of inositol 1,4,5-trisphosphate in homogenates of the cellular slime mould Dictyostelium discoideum.
Dictyostelium discoideum homogenates contain phosphatase activity which rapidly dephosphorylates Ins(1,4,5)P3 (D-myo-inositol 1,4,5-trisphosphate) to Ins (myo-inositol). When assayed in Mg2+, Ins(1,4,5)P3 is dephosphorylated by the soluble Dictyostelium cell fraction to 20% Ins(1,4)P2 (D-myo-inositol 1,4-bisphosphate) and 80% Ins(4,5)P2 (D-myo-inositol 4,5-bisphosphate). In the particulate frac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1961
ISSN: 0022-1287
DOI: 10.1099/00221287-25-3-375