Cubic lipid phase and bicelle crystallization of membrane proteins
نویسندگان
چکیده
منابع مشابه
Atomic Resolution Structures of Integral Membrane Proteins Using Cubic Lipid Phase Crystallization
The determination of high-resolution structural models of macromolecules, obligatory for detailed structure–function studies, requires large amounts of pure, stable target protein. The two principal methods of structure determination are X-ray crystallography, a method that requires growing highly ordered 3D crystals of the target protein, and nuclear magnetic resonance (NMR) spectroscopy, a me...
متن کاملCrystallization screens: compatibility with the lipidic cubic phase for in meso crystallization of membrane proteins.
The in meso method for growing crystals of membrane proteins uses a spontaneously forming lipidic cubic mesophase. The detergent-solubilized protein is dispersed with lipid, typically monoolein, and in so doing the cubic phase self-assembles. A precipitant is added to trigger crystal nucleation and growth. The commercial screen solution series are convenient for use in crystallization trials. T...
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Hydrated monoolein forms the cubic-Pn3m mesophase that has been used for in meso crystallization of membrane proteins. The crystals have subsequently provided high-resolution structures by crystallographic means. It is possible that the hosting cubic phase created by monoolein alone, which itself is not a common membrane component, will limit the range of membrane proteins crystallizable by the...
متن کاملHigh-throughput crystallization of membrane proteins using the lipidic bicelle method.
Membrane proteins (MPs) play a critical role in many physiological processes such as pumping specific molecules across the otherwise impermeable membrane bilayer that surrounds all cells and organelles. Alterations in the function of MPs result in many human diseases and disorders; thus, an intricate understanding of their structures remains a critical objective for biological research. However...
متن کاملRenaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic
Membrane proteins play vital roles in the life of the cell and are important therapeutic targets. Producing them in large quantities, pure and fully functional is a major challenge. Many promising projects end when intractable aggregates or precipitates form. Here we show how such unfolded aggregates can be solubilized and the solution mixed with lipid to spontaneously self-assemble a bicontinu...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2010
ISSN: 0108-7673
DOI: 10.1107/s0108767310093323