CTP:phosphocholine cytidylyltransferase activity in the preimplantation mouse embryo
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چکیده
منابع مشابه
Glucose 6-phosphate-dehydrogenase activity in the preimplantation mouse embryo.
1. The activity of glucose 6-phosphate dehydrogenase was determined in mouse embryos during the first 5 days of development from the time of ovulation up to implantation. 2. The activity decreased from 1.39 to 0.19mmumoles of NADP reduced/hr./embryo from ovulation to implantation. 3. The specific activity of the embryo on the first day was higher than specific activities reported for most other...
متن کاملAlkaline phosphatase activity in the preimplantation mouse embryo.
Alkaline phosphatase (AP) activity has been assayed in frozen sections of preimplantation mouse embryos by an azo-dye cytochemical method. The results indicate that during preimplantation mouse development AP activity is first expressed between the 8- and 16-cell stages and develops in all cells by the late morula stage. During blastocyst formation AP activity is lost or greatly reduced in trop...
متن کاملpreimplantation mouse embryo
The role of membrane sterols in the compaction and subsequent development of the preimplantation mouse embryo was studied by incubating embryos in 7-ketocholesterol and other oxygenated sterols. These sterols have been shown to inhibit sterol synthesis and deplete membranes of cholesterol in a variety of other cell types. Compaction and subsequent blastocyst formation were normal when embryos w...
متن کاملThe RNA polymerase activity of the preimplantation mouse embryo.
The activation of the mouse embryo genome has been studied during early cleavage, in vivo. Individual embryos, prepared as whole mounts, were assayed for endogenous RNA polymerase activity. RNA synthesis was detected by autoradiography as the incorporation of [3H]UMP into an acid-insoluble product. No RNA polymerase activity could be detected in the pronuclei of one-cell embryos. Radioactive in...
متن کاملDNA synthesis in the preimplantation mouse embryo.
Strain PO preimplantation embryos were labelled with [H]thymidine. The incorporation of the label was studied by autoradiography of air-dried and serially sectioned embryos. DNA amounts were measured with a microdensitometer. The following observations were made at the 5to 16-cell stages. All nuclei contained 2C-4C amounts of DNA and all could eventually synthesize DNA. However, after short lab...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1997
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.1100213