منابع مشابه
Crystalline Trypsin
A method is described for isolating a crystalline protein of high tryptic activity from beef pancreas. The protein has constant proteolytic activity and optical activity under various conditions and no indication of further fractionation could be obtained. The loss in activity corresponds to the decrease in native protein when the protein is denatured by heat, digested by pepsin, or hydrolyzed ...
متن کاملInactivation of Crystalline Trypsin
1. The rate of inactivation of crystalline trypsin solutions and the nature of the products formed during the inactivation at various pH at temperatures below 37 degrees C. have been studied. 2. The inactivation may be reversible or irreversible. Reversible inactivation is accompanied by the formation of reversibly denatured protein. This denatured protein exists in equilibrium with the native ...
متن کاملCrystalline Soybean Trypsin Inhibitor
A study has been made of the general properties of crystalline soybean trypsin inhibitor. The soy inhibitor is a stable protein of the globulin type of a molecular weight of about 24,000. Its isoelectric point is at pH 4.5. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly...
متن کاملCrystalline Chymo-trypsin and Chymo-trypsinogen
A new crystalline protein, chymo-trypsinogen, has been isolated from acid extracts of fresh cattle pancreas. This protein is not an enzyme but is transformed by minute amounts of trypsin into an active proteolytic enzyme called chymo-trypsin. The chymo-trypsin has also been obtained in crystalline form. The chymo-trypsinogen cannot be activated by enterokinase, pepsin, inactive trypsin, or calc...
متن کاملAntifatty Liver Activity of Crystalline Trypsin
The fatty liver that develops in insulin-treated, depancreatized dogs and in dogs subjected to ligation of their pancreatic ducts has been the subject of considerable study (1). Soon after its discovery it was shown to be prevented by the feeding of raw pancreas (2), while later findings revealed that it could also be prevented by the feeding of pancreatic juice and certain fractions derived fr...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1932
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.16.2.339